BioEPS

Courses

qPCR Basic Module (3 days)
Begin: 04.10.10, 09:00
End: 06.10.10, 16:30
Closing date: 27.09.10, 12:00
Free spaces: 7

High resolution melt - HRM (1 day)
Begin: 07.10.10, 09:00
End: 07.10.10, 16:30
Closing date: 30.09.10, 12:00
Free spaces: 8

qPC-R - data analysis using R packages (2 days)
Begin: 11.10.10, 09:00
End: 12.10.10, 16:30
Closing date: 04.10.10, 12:00
Free spaces: 9

Single-cell/qPCR Module (3 days)
Begin: 18.10.10, 09:00
End: 20.10.10, 16:30
Closing date: 11.10.10, 12:00
Free spaces: 7

Experiment Design and qPCR data processing (2 days)
Begin: 21.10.10, 09:00
End: 22.10.10, 16:30
Closing date: 14.10.10, 12:00
Free spaces: 14

Description

The course focus on experimental setup and data analysis doing RT-qPCR in samples with low nucleic acid amounts and even single-cells. Practical and theoretical parts. In English. Fees (+19% VAT): Academic (840€), Industry (1140€). Lunches and "Get together" dinner are included in the course fees.

Title:	Single-cell/qPCR Module (3 days)
Number:	25/10
State:	Not exceeded
Begin:	Monday, 18 October 2010 09:00
End:	Wednesday, 20 October 2010 16:30
Closing date:	Monday, 11 October 2010 12:00
Tutor:	Dr. M. Reiter, Dr. M. Pfaffl, Petra Hartmann, Antje Plaschke-Schlütter
Target group:	Beginner and advanced qPCR user
Location:	BioEPS GmbH Lise-Meitner-Strasse 30 85354 Freising Germany
Bookable:	7
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Day 1 Introduction into Methods for single-cell sorting Single-cell theory and applications Sampling and cultivation technologies Basic qPCR theory and applications Day 2: Principles of cDNA synthesis Pre-amplification Primer and Probe design Optimizations and experimental setup using single-cells Data analysis in single-cell experiments Day 3: Introduction to quantification principles Absoulte quantification with single-cells Relative quantification: Gene expression Calculations using different relative quantification methods Strategies for normalization of QPCR data Practical Part: In the practical part laser microdissection using the CellEctor system (MMI) will be presented from tissue material. Interested participants are encouraged to bring their personal sample material. Furhtermore the AmpliSpeed single-cell RT-PCR system from Advalytix (Beckman Coulter) will be used. Fees (incl. 19% VAT): Academic: 1047.20€ Industry: 1451.80€ For combined course modules (Single cell+qPCR data processing) we offer 200€ discount
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