| Description |
| Introductory course consisting of a theoretical and a practical part.
Issues: qPCR introduction, assay optimization, sampling and extraction methods, data analysis and quantification strategies. In English
Fees (+19% VAT): Academic (880€), Industry (1220€).
Lunches and "Get together" dinner are included in the course fees. |
| Title: |
qPCR Basic Module (3 days) |
| Number: |
21/10 |
| State: |
Not exceeded |
| Begin: |
Monday, 04 October 2010 09:00 |
| End: |
Wednesday, 06 October 2010 16:30 |
| Closing date: |
Monday, 27 September 2010 12:00 |
| Tutor: |
Dr. M. Reiter, Dr. M. Pfaffl |
| Target group: |
Beginner and advanced qPCR user |
| Location: |
BioEPS GmbH
Lise-Meitner-Strasse 30
85354 Freising
Germany |
| Bookable: |
7 |
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The introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains:
Day 1
- Basic PCR theory and applications
- Detection Chemistry (SYBR Green I, TaqMan, Molecular Beacons...etc)
- Different instrument platforms and their typical uses
- Primer Design, Probe design and optimization
- Basic data handling and analysis
Day 2:
- Experimental setup and how it influences qPCR data
- Nucleic acid extraction
- cDNA synthesis and pre-amplification
- Introduction to specific qPCR applications: genotyping, SNP analysis, HRM
Day 3:
- Introduction to quantification principles
- Quantification strategies and qPCR efficiency
- Absolute quantification
- Relative quantification
- Strategies for normalization of QPCR data
About the tutors:
Dr. Michael Pfaffl is one of the leading experts in qPCR data analysis, specialiced on relative quantification strategies. He is author of many highly influental publications.
Dr. Martina Reiter is qPCR specialist with focus on experiment setup and many years of experience in the optimization of qPCR experiments.
Fees (incl. 19% VAT):
Academic: 1047.20€
Industry: 1451.80€
For combined course modules (Basic+HRM) we offer 200€ discount!
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